Recombinant:Article Title: Incorporation of human iPSC-derived stromal cells creates a pancreatic cancer organoid with heterogeneous cancer-associated fibroblasts.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples the Human Pancreas cancer tissue array for antibody screening Biomax Cat#PA-1003 .. PCO-1 Pancreatic ductal adenocarcinoma patient-derived organoid N/A PCO-2 Pancreatic ductal adenocarcinoma patient-derived organoid N/A PCO-3 Pancreatic ductal adenocarcinoma patient-derived organoid N/A PCO-4 Pancreatic ductal adenocarcinoma patient-derived organoid N/A Chemicals, peptides, and recombinant proteins DMEM/Ham’s F-12 with L-Gln and Sodium Pyruvate, without HEPES, liquid nacalai tesque Cat#11581-15 Penicillin-Streptomycin Mixed Solution nacalai tesque Cat#26253-84 Dimethyl Sulfoxide nacalai tesque Cat#IW-13445-74 5-Fluorouracil nacalai tesque Cat#16220-14 Polyoxyethylene Sorbitan Monolaurate nacalai tesque Cat#35624-15 DAPI Solution(1 mg/mL) nacalai tesque Cat19178-91 D-PBS( ) without Ca and Mg, liquid Animal-Free nacalai tesque Cat#14249-24 GlutaMAXTM Supplement Thermo Fisher Scientific Cat#35050061 B-27TM Supplement (50X) Thermo Fisher Scientific Cat#17504001 StemProTM-34 SFM (1X) Thermo Fisher Scientific Cat#10639-011 Trypsin-EDTA (0.05%), phenol red Thermo Fisher Scientific Cat#25300-054 TrypLETM Express Enzyme (1X), no phenol red Thermo Fisher Scientific Cat#12604013 Serum, Fetal Bovine, BSE Tested, EC Approved CFBSD Thermo Fisher Scientific Cat#S1820 Lot#42F7686K Nicotinamide Sigma-Aldrich Cat#N-3376 N-acetyl-L-cysteine Sigma-Aldrich Cat#A-7250 hEGF Sigma-Aldrich Cat#E9644 Gastrin I human,R95% (HPLC) Sigma-Aldrich Cat#G9020 Bovine Serum Albumin 10% Solution in DPBS Sigma-Aldrich Cat#A1595 Gelatin from porcine skin Sigma-Aldrich Cat#G1890 Poly-L-lysine solution Sigma-Aldrich Cat#P4832 Collagenase Sigma-Aldrich Cat#C5138 Noggin, Human,Recombinant Peprotech Cat#120-10C FGF10,Human, Recombinant Peprotech Cat#100-26 R-spondin,Human, Recombinant Peprotech Cat#120-38 PDGF-BB, Human, Recombinant, Animal Free Peproteh Cat#AF-100-14B A-83-01 Selleck chem Cat#S-7692 Recombinant Human Wnt-3a R&D Systems Cat#5036-WN-500 BMP-4, Human, Recombinant R&D Systems Cat#314-BP-010 EGM Bullet kit Lonza Cat#CC-3124 CultureSure Y-27632 Fujifilm Cat#034-24024 D-MEM(High Glucose) with L-Glutamine, Phenol Red and Sodium Pyruvate Fujifilm Cat#043-30085 Vascular Endothelial Growth Factor(VEGF-A165), Human, recombinant Fujifilm Cat#229-01313 (Continued on next page) Cell Reports 42, 113420, November 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fibroblast Growth Factor (basic)) (FGF-basic/bFGF/FGF2), Human, recombinant(154aa) Fujifilm Cat#060-04543 4% Paraformaldehyde Phosphate Buffer Solution Fujifilm Cat#163-20145 Sucrose Fujifilm Cat#196-00015 Apathy’s Mounting Media, Soluble Fujifilm Cat#010-13811 Sodium Acetate Fujifilm Cat#195-13915 Corning Matrigel Basement Membrane Matrix, LDEV-free CORNING Cat#354234 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free, 10 mL CORNING Cat#354230 StemFit AK02N Ajinomoto Cat#AK02N Recombinant human Activin A Ajinomoto Cat#SP-ActA-R StemFit For Differentiation Ajinomoto Cat#AS401 MiraCell EC Culture Medium Takara Bio Cat#DS-Y50053 CHIR99021 Cayman Chemical Company Cat#13122 Forskolin Cayman Chemical Company Cat#11018 Accutase Innnovative Cell Technologies Inc Cat#AT104-500 iMatrix-511 silk Matrixome Cat#892021 CELLBANKER 1 ZENOAQ Cat#CB011 STEM-CELLBANKER GMP grade ZENOAQ Cat#CB045 Paclitaxel MedChemExpress Cat#HY-B0015 Gemzar Injection Eli Lilly and Company N/A Protein Block, Serum-Free, Liquid form Dako Cat#X090930-2 Glo lysis buffer, 1X Promega Cat#E2661 Mayer’s Hematoxylin Solution for nuclear staining MUTO PURE CHEMICALS Cat#30011 Pure Eosin solution MUTO PURE CHEMICALS Cat#32041 1% Sirius red solution MUTO PURE CHEMICALS Cat#3306-1 Accumax M&S TechnoSystems Cat#AM105-500ML PI IMMUNOSTEP Cat#PI Pronase Roche Cat#10165913103 Critical commercial assays ONE-GloTM Luciferase Assay System Promega Cat#E6120 Nano-Glo Luciferase Assay System Promega Cat#N1120 ProFection Mammalian Transfection System Promega Cat#E1200 Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 Fibronectin ElA Kit Takara Bio Cat#MK115 Wash and Stop Solution for ELISA without Sulfuric Acid Takara Bio Cat#MK021 Hyaluroan Quantikine ELISA Kit R&D Systems Cat#DHYAL0 PureLink RNA Mini Kit Thermo Fisher Scientific Cat#12183018A PureLink RNA Micro Scale Kit Thermo Fisher Scientific Cat#12183016 RNA ScreenTape Agilent technologies Cat#5067-5576 RNA Sample Buffer Agilent technologies Cat#5067-5577 High Sensitivity D5000 ScreenTape Agilent technologies Cat#5067-5592 High Sensitivity D5000 Reagents Agilent technologies Cat#5067-5593 (Continued on next page) 16 Cell Reports 42, 113420, November 28, 2023
High Performance Liquid Chromatography:Article Title: Incorporation of human iPSC-derived stromal cells creates a pancreatic cancer organoid with heterogeneous cancer-associated fibroblasts.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples the Human Pancreas cancer tissue array for antibody screening Biomax Cat#PA-1003 .. PCO-1 Pancreatic ductal adenocarcinoma patient-derived organoid N/A PCO-2 Pancreatic ductal adenocarcinoma patient-derived organoid N/A PCO-3 Pancreatic ductal adenocarcinoma patient-derived organoid N/A PCO-4 Pancreatic ductal adenocarcinoma patient-derived organoid N/A Chemicals, peptides, and recombinant proteins DMEM/Ham’s F-12 with L-Gln and Sodium Pyruvate, without HEPES, liquid nacalai tesque Cat#11581-15 Penicillin-Streptomycin Mixed Solution nacalai tesque Cat#26253-84 Dimethyl Sulfoxide nacalai tesque Cat#IW-13445-74 5-Fluorouracil nacalai tesque Cat#16220-14 Polyoxyethylene Sorbitan Monolaurate nacalai tesque Cat#35624-15 DAPI Solution(1 mg/mL) nacalai tesque Cat19178-91 D-PBS( ) without Ca and Mg, liquid Animal-Free nacalai tesque Cat#14249-24 GlutaMAXTM Supplement Thermo Fisher Scientific Cat#35050061 B-27TM Supplement (50X) Thermo Fisher Scientific Cat#17504001 StemProTM-34 SFM (1X) Thermo Fisher Scientific Cat#10639-011 Trypsin-EDTA (0.05%), phenol red Thermo Fisher Scientific Cat#25300-054 TrypLETM Express Enzyme (1X), no phenol red Thermo Fisher Scientific Cat#12604013 Serum, Fetal Bovine, BSE Tested, EC Approved CFBSD Thermo Fisher Scientific Cat#S1820 Lot#42F7686K Nicotinamide Sigma-Aldrich Cat#N-3376 N-acetyl-L-cysteine Sigma-Aldrich Cat#A-7250 hEGF Sigma-Aldrich Cat#E9644 Gastrin I human,R95% (HPLC) Sigma-Aldrich Cat#G9020 Bovine Serum Albumin 10% Solution in DPBS Sigma-Aldrich Cat#A1595 Gelatin from porcine skin Sigma-Aldrich Cat#G1890 Poly-L-lysine solution Sigma-Aldrich Cat#P4832 Collagenase Sigma-Aldrich Cat#C5138 Noggin, Human,Recombinant Peprotech Cat#120-10C FGF10,Human, Recombinant Peprotech Cat#100-26 R-spondin,Human, Recombinant Peprotech Cat#120-38 PDGF-BB, Human, Recombinant, Animal Free Peproteh Cat#AF-100-14B A-83-01 Selleck chem Cat#S-7692 Recombinant Human Wnt-3a R&D Systems Cat#5036-WN-500 BMP-4, Human, Recombinant R&D Systems Cat#314-BP-010 EGM Bullet kit Lonza Cat#CC-3124 CultureSure Y-27632 Fujifilm Cat#034-24024 D-MEM(High Glucose) with L-Glutamine, Phenol Red and Sodium Pyruvate Fujifilm Cat#043-30085 Vascular Endothelial Growth Factor(VEGF-A165), Human, recombinant Fujifilm Cat#229-01313 (Continued on next page) Cell Reports 42, 113420, November 28, 2023 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fibroblast Growth Factor (basic)) (FGF-basic/bFGF/FGF2), Human, recombinant(154aa) Fujifilm Cat#060-04543 4% Paraformaldehyde Phosphate Buffer Solution Fujifilm Cat#163-20145 Sucrose Fujifilm Cat#196-00015 Apathy’s Mounting Media, Soluble Fujifilm Cat#010-13811 Sodium Acetate Fujifilm Cat#195-13915 Corning Matrigel Basement Membrane Matrix, LDEV-free CORNING Cat#354234 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix, LDEV-free, 10 mL CORNING Cat#354230 StemFit AK02N Ajinomoto Cat#AK02N Recombinant human Activin A Ajinomoto Cat#SP-ActA-R StemFit For Differentiation Ajinomoto Cat#AS401 MiraCell EC Culture Medium Takara Bio Cat#DS-Y50053 CHIR99021 Cayman Chemical Company Cat#13122 Forskolin Cayman Chemical Company Cat#11018 Accutase Innnovative Cell Technologies Inc Cat#AT104-500 iMatrix-511 silk Matrixome Cat#892021 CELLBANKER 1 ZENOAQ Cat#CB011 STEM-CELLBANKER GMP grade ZENOAQ Cat#CB045 Paclitaxel MedChemExpress Cat#HY-B0015 Gemzar Injection Eli Lilly and Company N/A Protein Block, Serum-Free, Liquid form Dako Cat#X090930-2 Glo lysis buffer, 1X Promega Cat#E2661 Mayer’s Hematoxylin Solution for nuclear staining MUTO PURE CHEMICALS Cat#30011 Pure Eosin solution MUTO PURE CHEMICALS Cat#32041 1% Sirius red solution MUTO PURE CHEMICALS Cat#3306-1 Accumax M&S TechnoSystems Cat#AM105-500ML PI IMMUNOSTEP Cat#PI Pronase Roche Cat#10165913103 Critical commercial assays ONE-GloTM Luciferase Assay System Promega Cat#E6120 Nano-Glo Luciferase Assay System Promega Cat#N1120 ProFection Mammalian Transfection System Promega Cat#E1200 Tumor Dissociation Kit, human Miltenyi Biotec Cat#130-095-929 Fibronectin ElA Kit Takara Bio Cat#MK115 Wash and Stop Solution for ELISA without Sulfuric Acid Takara Bio Cat#MK021 Hyaluroan Quantikine ELISA Kit R&D Systems Cat#DHYAL0 PureLink RNA Mini Kit Thermo Fisher Scientific Cat#12183018A PureLink RNA Micro Scale Kit Thermo Fisher Scientific Cat#12183016 RNA ScreenTape Agilent technologies Cat#5067-5576 RNA Sample Buffer Agilent technologies Cat#5067-5577 High Sensitivity D5000 ScreenTape Agilent technologies Cat#5067-5592 High Sensitivity D5000 Reagents Agilent technologies Cat#5067-5593 (Continued on next page) 16 Cell Reports 42, 113420, November 28, 2023
other:
Article Title: Controlled aggregative assembly to form self-organizing macroscopic human intestine from induced pluripotent stem cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Recombinant murine EGF Peprotech Cat#31509 Recombinant mouse Noggin R&D Cat#1967 Recombinant mouse R-spondin1 R&D Cat#3474-RS A83-01 Tocris Cat#2939 Recombinant Human IGF-1 BioLegend Cat#590906 Nicotinamide Sigma Cat#N0636 Afamin/Wnt3a conditioned medium MBL lifescience Cat#J2-001 Activin A Nacalai Tesque Cat#18585-81 CHIR99021 Cayman Chemicals Cat#13122 Recombinant human FGF4 Peprotech Cat#100-31 Recombinant human BMP-4 R&D Cat# 314-BP Wnt-C59 Chemscene LLC Cat#CS-1420 PIK90 Cayman Chemicals Cat#10010749 Retinoic acid Sigma Aldrich Cat#R2625 Purmorphamine Tocris Bioscience Cat#4551 Y-27632 Fujifilm Wako Pure Chemical Cat#253-00513 FGF-basic(154a.a.), Human, Recombinant Peprotech Cat#100-18B Recombinant Human IGF-I BioLegend Cat#590906 Cellartis DEF-CSTM 500 culture system Takara Bio Cat#Y50101 Advanced DMEM/F12 Thermo Fisher Cat#12634010 DMEM, high glucose Thermo Fisher Cat#11965-092 RPMI 1640 with L-Gln, liquid Nacalai Tesque Cat#30264-85 Embryonic stem cell Fetal Bovine Serum Thermo Fisher Cat#16141079 GlutaMAXTM Supplement Thermo Fisher Cat#35050-061 HEPES buffer Nacalai Tesque Cat#17557-94 Penicillin-Streptomycin Mixed Solution Nacalai Tesque Cat#26253-84 Matrigel Growth Factor Reduced Corning Cat#356231 N-2 supplement (100X) Thermo Fisher Cat#17502-048 B-27 Supplement (50X), serum free Thermo Fisher Cat#17504-044 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat#12587010 TrypLE Express Enzyme Thermo Fisher Cat#12604013 Accumax Nacalai Tesque Cat#17087-54 Hoechst 33342 Thermo Fisher Cat#H3570 VECTASHIELD mounting medium with DAPI VECTOR Laboratories Cat#H1200 DAPI solution (1 mg/mL) Nacalai Tesque Cat#19178-91 The P3 Primary Cell 4D-Nucleofector X Kit S Lonza Cat#V4XP-3032 Critical commercial assays RNAscope 2.5HD Assay-Red Advanced Cell Diagnostics Cat#322350 RNeasy Mini kit QIAGEN Cat#74106 QuantiTect Reverse Transcription Kit QIAGEN Cat#205313 Experimental models: Cell lines Human induced pluripotent stem cell line: HiPS-RIKEN-2F RIKEN BRC Cell Bank (Japan) HPS0014 Human induced pluripotent stem cell line: PB001 Kindly gifted by Dr Hideki Masaki (Institute of Medical Science, the University of Tokyo) N/A Human induced pluripotent stem cell line: TkdA3-4 Institute of Medical Science, the University of Tokyo N/A (Continued on next page) Cell Reports Methods 4, 100930, December 16, 2024 e2
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